Tuesday, March 17, 2009

Coomb's test


Assalamu'alaikum wbt

Just want to share about Coomb's test with u all guys...after have been lectured during hematology class yesterday...

Sharing is Loving~~

~Setiap Kesulitan Pasti Ada Kemudahan~

What is Coomb’s Test?

The test perform to detect incomplete antierythrocyte antibodies and cause premature RBC destruction (hemolysis).



Alternative names?

Direct antiglobulin test (DAT), and Indirect antiglobulin test (IAT)


What is normal result?

No clumping of cells (agglutination), indicating that there are no antibodies to red blood cells.

Direct Antiglobulin Test (DAT)

Indirect Antiglobulin Test (IAT)

To detect antibodies bound to RBC surface

To detect antierythrocyte antibodies free in serum


Patient’s blood

Patient’s serum


Procedure:

  • Obtain washed RBCs (to eliminate all plasma IgG)
  • Add antiglobulin antiserum
  • Incubate 15 min 37oC
  • Look for agglutination (visual, microscope)

Procedure:

  • Washed normal RBCs, group 0 (or compatible)
  • Incubate 15 min 37oC
  • Wash incubated RBCs (to eliminate serum IgG)
  • Add antiglobulin antiserum
  • Incubate 15 min 37oC
  • Look for agglutination (visual, microscope)

Usually performed to diagnosed the cause of anemia or jaundice

Rarely used to diagnose a medical condition. Often use to determine whether a person might have a reaction to a blood transfusion.


1 comments:

ainaa ismail on March 21, 2009 at 12:59 PM said...

thanks niza!

summary
1.DAT-guna patient's RBC and test on known antibody

2.IAT-guna patient's serum and test on known RBC

lalalala.thanks=p

 

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