Fluorescent screening test for G6PD deficiency

principle-
G6PD will produce NADPH from NADP+.These NADPHs will fluoresces under UV light.In G6PD deficiency,less fluoresces occur due to less NADPH production

in what case,the result is false?

• False normal

Reticulocyte has large amount of G6PD.Therefore a small num of reticulocytes will produce large num of NADPH,

• False deficient

In anaemic condition,very little fluoresces is seen,eventhough the G6PD IS NORMAL

source:FLM